Donmuş Çözünmüş Kanın Farklı Değerlerde Santrifüjünün DNA İzolasyonu Üzerine Etkileri

DNA isolation from blood is a commonly used application to obtain nDNA and mtDNA. It was previously shown that could be performed the pellet obtained after centrifugation of freeze-thawed (FTB), this pretreatment had constructive results on isolation. However, which levels can for pretreatment, their effects are unknown. The aim study determine appropriate show isolated DNA. For purpose, isolations were carried out supernatant by at different FTB. Then, spectrophotometric, gel electrophoresis, real-time PCR analyses in samples. As result, FTB 5,000×g 2 min or over let genetic material completely. This also caused high amount mtDNA/nDNA ratios did not change samples pellets defined levels, but integrity decreased. To conclude, harvest wash found before isolations.